Re: CD31 protocol
Here is the protocol we use for CD31.
CD31; clone 1A10
Mfgr/Vendor: Novocastra# NCL-CD31-1A10
Pretreatment: Steam in 10mM citrate buffer, pH 6.0(+/-0.3) for 20 minutes
in vegetable steamer, followed by a 20' cooldown. Wash in buffer.
Dilution: Apply at 1:200 and incubate for 40 minutes @RT. Wash in
Secondary: biotinylated anti-MOUSE IgG, Vector # BA-2000, 1:300 in PBS
(incubate 20¹ @ RT) Wash in buffer.
Detection: Elite Avidin-Biotin-Complex‹Horseradish Peroxidase (ABCHRP),
Vector # PK-6100, made per kit instructions. Incubate 20 minutes @RT.
Wash in buffer.
Best of luck!
PhenoPath Laboratories--Seattle, WA
on 4/19/02 1:21 AM, Sven Terclavers at Sven.Terclavers@med.kuleuven.ac.be
> Hi listers,
> Does anyone of you know of a protocol to stain CD31 without the use of
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