Re: Brazil's fluid
Tony Henwood has posted some information and a request for a reference
concerning Brazil's fixative. I append his entire post below.
Eleven years ago I corresponded with Arthur H. Cohen, M.D. in the Department
of Pathology, Division of Renal Pathology LAC-Harbor-UCLA Medical Center in
Torrance CA, and I still have the correspondence on file. I asked him:
I just read your article "Frozen-section analysis of allograft renal biopsy
specimens: reliable histopathologic data for rapid decision making" in Arch
Pathol Lab Med 1991;115:386-389; April 1991. You refer to your article
"Masson's trichrome stain in the evaluation of renal biopsies: an appraisal"
in Am J Clin Pathol 1976;65:631-643 which apparently contains your formula
for alcoholic Bouin's solution, in which you fixed renal biopsy specimens for
at least an hour before frozen section.
He replied: I am enclosing the recipe for alcoholic Bouin's fixative which is
also known as Dubosq-Brazil [Duboscq-Brazil] fixative. Stains commonly
employed in renal pathology (Masson's trichrome, periodic acid-methenamine
silver) come out especially well.
80% ethanol 150 mL
37% formaldehyde 60 mL
picric acid 1 g
Add 1 mL glacial acetic acid to 14 mL stock solution before use.
Leave in the fixative from 6 to 24 hours depending on the size of the tissue.
Transfer to 70% alcohol to await processing for light microscopy.
Reference: Histopathology Laboratory Procedures, National Cancer Institute
Samurai pathologist's note: To avoid handling solid picric acid, since
saturated aqueous picric acid contains about 21 g/L of picric acid, 30 mL of
it would contain about 600 mg. If one amended this formula to:
saturated aqueous picric acid 30 mL
absolute ethanol 120 mL
37% formaldehyde 60 mL
one would get a mixture that contained about 60% of the amount of picric acid
present in the previous formula.
The only other reference that I [the Samurai Pathologist, that is] could
locate was in The microtomist's vade-mecum (Bolles Lee): a handbook of the
methods of animal and plant microscopic technique. 11th ed. Gatenby JB and
Beams HW, eds. Blakiston, Philadelphia, 1950. p. 325. This venerable
reference spell it Duboscq-Brazil.
My laboratory has used a fixative called BRAZIL'S FLUID for fixing tissues
where small amounts of glycogen are being sought. It is supposedly a good
fixative for glycogen. Unfortunately I have no reference for this fixative
nor its uses. The formula is as follows:
Absolute alcohol 1650ml
Trichloracetic acid 7.5g
Picric acid 10g
Dissolve picric acid, then Trichloracetic acid in the alcohol, then add the
Normal block size 3-4 mm thick 24 hours
Needle biopsy 3-4 hours
The only one I know of is Dubosq-Brasil fluid which is similar (an alcoholic
ethyl alcohol 80% 150ml
picric acid 1g
immediately before use add:
Formol 35% 60ml
acetic acid, pure 15ml.
From: Bock, P.: "Romeis Mikroskopische Technik", 17th. ed., p. 98.
Would anyone have a reference for BRAZIL'S FLUID that they could share with
Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318
<< Previous Message | Next Message >>