Re: Black Gold

From:"J. A. Kiernan"

"Connolly, Brett M" wrote:
> Isn't that also known as "Texas Tea"...oil, that is? Check out a
> company called Histo-Chem, Inc.

This is another of L. C. Schmued's  compounds, and
like his fluoro-jade the identity is a trade secret.
The web site says it's a "haloaurophosphate" and provides
technical instructions for its use.

BUT why use this expensive unknown substance to stain
myelin black when the job can be done inexpensively either
with sudan black B on frozen sections or with Loyez's
iron haematoxylin on paraffin sections? (Palladium chloride
also works nicely on frozen sections of peripheral tissues
[but it's not so good for the CNS]).  
--                                      John
Andrea Grantham had asked:
>> Is there anybody in histoland doing a stain on brain tissue for myelin
>> called Black Gold. I'm looking for a protocol. I have a journal article
>> from the investigator who is requesting the stain but the directions are
>> not real clear.
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1

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