Re: Adenovirus

From:Richard Cartun

I have noticed that HIER may reduce adenovirus immunoreactivity;
however, that observation may pertain to the antibody used in our
laboratory (Chemicon MAB850) and not the virus itself.  We use pepsin
digestion for our pretreatment.

R. Cartun

>>> Jenny Oblander  04/17/02
11:20AM >>>
	 I am getting ready to stain FFPE lung for adenovirus. Is there
tricks or potential glitches I should know in advance? I will be using
mouse monoclonal IgG. They do recommend using trypsin, has anyone used
proteinase K instead. I don't want to waste any of this antibody, it
mucho expensive. So any and all help will be appreciated.  Thanks

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