Re: Adenovirus
I have noticed that HIER may reduce adenovirus immunoreactivity;
however, that observation may pertain to the antibody used in our
laboratory (Chemicon MAB850) and not the virus itself. We use pepsin
digestion for our pretreatment.
R. Cartun
>>> Jenny Oblander 04/17/02
11:20AM >>>
Hi,
I am getting ready to stain FFPE lung for adenovirus. Is there
any
tricks or potential glitches I should know in advance? I will be using
a
mouse monoclonal IgG. They do recommend using trypsin, has anyone used
proteinase K instead. I don't want to waste any of this antibody, it
is
mucho expensive. So any and all help will be appreciated. Thanks
Jenny
<< Previous Message | Next Message >>