RE: paraffin sectioning question

From:"Bartlett, Jeanine"

I used to use agar (2%) but now find that I prefer Histogel from

Jeanine Bartlett, HT(ASCP)
Centers for Disease Control
Infectious Disease Pathology Activity
1600 Clifton Rd., N.E.  MS-G32
Atlanta, GA  30333 

-----Original Message-----
From: April E. Monchik []
Sent: Thursday, April 18, 2002 9:20 AM
Subject: paraffin sectioning question

I'm trying to process, embed, and section in paraffin cultured cells 
called HL60s. I am having trouble getting the cells to pellet 
cohesively.  Does anyone have  any ideas on how to section a cell 
pellet in paraffiin?  I understand I need to embed the pellet in 
gelatin  first, but what percentage is used?  Any helpful hints or 
ideas would be greatly appreciated.
  - April

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