RE: paraffin sectioning question
I used to use agar (2%) but now find that I prefer Histogel from
Richard-Allan.
Jeanine Bartlett, HT(ASCP)
Centers for Disease Control
Infectious Disease Pathology Activity
1600 Clifton Rd., N.E. MS-G32
Atlanta, GA 30333
-----Original Message-----
From: April E. Monchik [mailto:aem2002@med.cornell.edu]
Sent: Thursday, April 18, 2002 9:20 AM
To: histonet@pathology.swmed.edu
Subject: paraffin sectioning question
Hi,
I'm trying to process, embed, and section in paraffin cultured cells
called HL60s. I am having trouble getting the cells to pellet
cohesively. Does anyone have any ideas on how to section a cell
pellet in paraffiin? I understand I need to embed the pellet in
gelatin first, but what percentage is used? Any helpful hints or
ideas would be greatly appreciated.
- April
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