|From:||Tom McNemar |
Tom Mc Nemar HT(ASCP)
Licking Memorial Hospital
From: eileen dusek [mailto:email@example.com]
Sent: Thursday, April 18, 2002 12:59 PM
To: firstname.lastname@example.org; email@example.com
Subject: Re: c-kit (CD117)>From: "Klassen, Shannon SDH"
We have been successful with Dako's CD117 using it at 1:100 with out any treatment.
Our control tissue is a gastrointestinal stromal tumor.
I hope this helps.
Eileen C. Dusek
Central DuPage Hospital
>To: 'Histonet' >Subject: c-kit (CD117) >Date: Thu, 18 Apr 2002 09:55:52 -0600 > >Can someone out there in histoland help me get this antibody working - >Novocastra ckit clone T595 - I'm not getting membrane staining - some >cytoplasmic and some almost appears nuclear. I've tried a variety of >different tumors - GIST, melanoma, breast , normal skin and haven't had any >success. >Down to 1\10 with antigen retrieval in citrate buffer in microwave. >HEELLLPPP!! > >Shannon Klassen >IHC Tech >Saskatoon Health District >Saskatoon, Canada >
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