Brett,
Yes, it worked beautifully. We were considering it as an alternative to
BrDU, but it does not mark all stages of mitosis so it was a no go for us.
 I used HIER in citrate buffer-steamer combo for 20 on, 20 cool. Used
Upstate Biotechnology #06-570 Polyclonal ab 1:100 at room temp for 60 min.
Secondary at 1:50 for 30 min, then ABC-DAB , results  were lovely on both
rat and mouse. If you want specifics give me a shout.
Good luck!

Mary Georger
Center for Cardiovascular Research
University of Rochester Medical Center
KMRB Room 2-9816
601 Elmwood Avenue.
PO Box 679
Rochester, New York  14642
585-273-1548

> ----------
> From: 	Connolly, Brett M
> Sent: 	Friday, April 19, 2002 10:40 AM
> To: 	'HISTONET' (E-mail)
> Subject: 	anti-phospho-Histone 3
> 
> Hi all,
> Has anyone tried staining FFPE section with an anti-phospho-Histone 3
> antibody??
> Brett
> 
> Brett M. Connolly, Ph.D.
> Merck Research Laboratories
> Dept. of Neuroscience
> WP26A-3000
> PO Box 4
> West Point, PA 19486
> PH 215-652-2501
> fax. 215-652-2075
> e-mail. brett_connolly@merck.com
> 
> 
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