I used
Paraclear and now Citrosolv on my stain line and processor. I only use
Xylene on the purging cycle of the processor to remove the paraffin in the
tubing/chambers better. Both Para clear and Citrosolv works good and are
compatible with most mounting media and other processing and staing
reagents. Hey, you have my first name!
- Ian R. Bernard, SSgt, USAF, AAS, HT
(ASCP)
- NCOIC Histopathology/ Pathobiology
Laboratory
- 59th Clinical Research Sqd / MSROP
- 1255 Wilford Hall Loop, bldg 4430
- Lackland AFB, Tx 78236-5319
- Tel: 210-292-7190
- Fax: 210-292-6053
- E-mail:
Ian.bernard@59mdw.whmc.af.mil
The
forces of darkness are moving on me and insist I move away from xylene. So,
from the many substitutes on the market what is the current favourite?
Although I'd like to hear all comments those from the UK would be very
welcome, just for ease of supply. At the moment I use Tissue-Clear (Sakura)
and mount from xylene using Entellan. Are any of these substitutes miscible
with Entellan, DPX and Balsam etc. or is it the 'own brand
mountant'.