RE: Xylene substitutes.

From:Bernard Ian R SSgt 59 CRES/MSROP

I used Paraclear and now Citrosolv on my stain line and processor.  I only use Xylene on the purging cycle of the processor to remove the paraffin in the tubing/chambers better.  Both Para clear and Citrosolv works good and are compatible with most mounting media and other processing and staing reagents.  Hey, you have my first name!
Ian R. Bernard, SSgt, USAF, AAS, HT (ASCP)
NCOIC Histopathology/ Pathobiology Laboratory
59th Clinical Research Sqd / MSROP
1255 Wilford Hall Loop, bldg 4430
Lackland AFB, Tx 78236-5319
Tel: 210-292-7190
Fax: 210-292-6053
-----Original Message-----
From: Dr. Ian Montgomery []
Sent: Friday, April 26, 2002 9:31 AM
Subject: Xylene substitutes.

        The forces of darkness are moving on me and insist I move away from xylene. So, from the many substitutes on the market what is the current favourite? Although I'd like to hear all comments those from the UK would be very welcome, just for ease of supply. At the moment I use Tissue-Clear (Sakura) and mount from xylene using Entellan. Are any of these substitutes miscible with Entellan, DPX and Balsam etc. or is it the 'own brand mountant'.

Dr. Ian Montgomery,
Microscopy Service Unit,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
G12 8QQ.
Tel: 0141 332 8855
Office: 4652
Lab: 6644.

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