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I wanted to get my tech's fingers out of xylene as it is  supposed  to be a
neuro-toxin. We use Surgipath's Clearium to coverslip out of isopropyl
alcohol. It works fine and no fading after years. (pathologists never
noticed)

-----Original Message-----
From: Dr. Ian Montgomery [mailto:ian.montgomery@bio.gla.ac.uk]
Sent: Friday, April 26, 2002 10:31 AM
To: histonet@pathology.swmed.edu
Subject: Xylene substitutes.


        The forces of darkness are moving on me and insist I move away from
xylene. So, from the many substitutes on the market what is the current
favourite? Although I'd like to hear all comments those from the UK would be
very welcome, just for ease of supply. At the moment I use Tissue-Clear
(Sakura) and mount from xylene using Entellan. Are any of these substitutes
miscible with Entellan, DPX and Balsam etc. or is it the 'own brand
mountant'.
Ian.
        . 


Dr. Ian Montgomery,
Microscopy Service Unit,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
Glasgow,
G12 8QQ.
Tel: 0141 332 8855 
Office: 4652
Lab: 6644.
e-mail: ian.montgomery@bio.gla.ac.uk 


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I 
wanted to get my tech's fingers out of xylene as it is  supposed  to 
be a neuro-toxin. We use Surgipath's Clearium to coverslip out of isopropyl 
alcohol. It works fine and no fading after years. (pathologists never 
noticed)

  
-----Original Message-----
From: Dr. Ian Montgomery 
  [mailto:ian.montgomery@bio.gla.ac.uk]
Sent: Friday, April 26, 2002 
  10:31 AM
To: histonet@pathology.swmed.edu
Subject: Xylene 
  substitutes.

        The 
  forces of darkness are moving on me and insist I move away from xylene. So, 
  from the many substitutes on the market what is the current favourite? 
  Although I'd like to hear all comments those from the UK would be very 
  welcome, just for ease of supply. At the moment I use Tissue-Clear (Sakura) 
  and mount from xylene using Entellan. Are any of these substitutes miscible 
  with Entellan, DPX and Balsam etc. or is it the 'own brand 
  mountant'.
Ian.
        . 
  

  
Dr. Ian Montgomery,
Microscopy Service 
  Unit,
Graham Kerr Building,
Institute of Biomedical & Life 
  Sciences,
University of Glasgow,
Glasgow,
G12 8QQ.
Tel: 0141 332 
  8855 
Office: 4652
Lab: 6644.
e-mail: 
  ian.montgomery@bio.gla.ac.uk 

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