RE: ? Problems with xylene and subs

From:"Smith, Allen"
I have occasionally mounted directly from absolute ethanol in Euparal.  Hematoxylins are fast in Euparal, but some counterstains are not. 
 

Allen A. Smith, Ph.D.
Barry University
School of Graduate Medical Sciences
    Podiatric Medicine and Surgery
Miami Shores, Florida  33161-6695

-----Original Message-----
From: Melissa Jensen [mailto:melzy@indytel.com]
Sent: Saturday, April 27, 2002 8:40 PM
To: histonet@pathology.swmed.edu
Subject: ? Problems with xylene and subs

We have a current dilemma in our lab..One prep tech had a reaction to the xylene that caused her massive headaches.When the xylene was removed and a sub used,her headaches went away.Now the other prep tech,while using substitute..clear right..and the mounting media that goes with is having alot of trouble..I mean she turns white!Nausea etc....Any suggestions?...The problem with substitutes is that not all mounting media is compatible..witch is a pain..ya have to wait a week or 2 fro a sample..etc..And we don't know if its the media or the sub! Also One pathologist refuses to have any citres smelling sub
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