RE: Grocott problems
Hi, some other things to try are 1. how much borax do you put into the
working solution? Too much will take forever to stain- try decreasing
amount or percentage. Or 2. how long ago were the control slides cut?
SOme bugs, like spirocetes, decrease stainablity over time. I don't know if
it's been documented for PCP but it happened to me once and that's what
fixed it for me. Good luck! Cheryl
>From: "Weems, Joyce"
>To: "'Johnston, Kathy'" , "Histonet (E-mail)"
>Subject: RE: Grocott problems
>Date: Fri, 26 Apr 2002 13:57:32 -0400
>1st of all - is your glass ware acid cleaned? 2nd - Do you have a neighbor
>that you could ask to do parallel stains - just to see if it might be
>something in the processing? If the same thing happens at your neighbor's,
>then you'll know it's not just the stain. Just my first thoughts.
>Saint Joseph's Hospital of Atlanta
> -----Original Message-----
> From: Johnston, Kathy [SMTP:Kathy.Johnston@CLS.ab.ca]
> Sent: Friday, April 26, 2002 1:34 PM
> To: Histonet (E-mail)
> Subject: Grocott problems
> We have been experiencing problems with our GMS stain over the past
> weeks. We run both a control slide containing fungal hyphae, and p.
> to cover our bases. Our problem is: it is taking much longer to
> silver, and after we tone, and Na thio the slides, they are so pale
> existent), that they are unacceptable.
> We have made new: 5% silver, 3% methenamine, 5% borax, stock silver
> methenamine, 5% chromic acid, 0.1% gold chloride, and 2% sodium
> to little avail other than to be able to make them acceptable to
> but not up to our usual standard.
> Any help on this very frustrating dilemma would be greatly
> Thanks in advance!
> Kathy Johnston
> Tech II - Special Stains
> Anatomic Pathology - Foothills Medical Center
> Calgary Laboratory Services
> Ph - 403-670-4760
> Fax - 403-270-4093
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