RE: Grocott problems
1st of all - is your glass ware acid cleaned? 2nd - Do you have a neighbor
that you could ask to do parallel stains - just to see if it might be
something in the processing? If the same thing happens at your neighbor's,
then you'll know it's not just the stain. Just my first thoughts.
Saint Joseph's Hospital of Atlanta
From: Johnston, Kathy [SMTP:Kathy.Johnston@CLS.ab.ca]
Sent: Friday, April 26, 2002 1:34 PM
To: Histonet (E-mail)
Subject: Grocott problems
We have been experiencing problems with our GMS stain over the past
weeks. We run both a control slide containing fungal hyphae, and p.
to cover our bases. Our problem is: it is taking much longer to
silver, and after we tone, and Na thio the slides, they are so pale
existent), that they are unacceptable.
We have made new: 5% silver, 3% methenamine, 5% borax, stock silver
methenamine, 5% chromic acid, 0.1% gold chloride, and 2% sodium
to little avail other than to be able to make them acceptable to
but not up to our usual standard.
Any help on this very frustrating dilemma would be greatly
Thanks in advance!
Tech II - Special Stains
Anatomic Pathology - Foothills Medical Center
Calgary Laboratory Services
Ph - 403-670-4760
Fax - 403-270-4093
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