Jim,
There was a great article in BioTechniques, Vol. 32, No.2 (2002) 296-298
about decal & Zebra Fish & Immunos.
If you can't access the article give me a shout with your Fax and I'll fax
it to you.
Good Luck!
Mary Georger
Center for Cardiovascular Research
University of Rochester Medical Center
KMRB Room 2-9816
601 Elmwood Avenue.
PO Box 679
Rochester, New York  14642
585-273-1548

> ----------
> From: 	Fessler, Jim
> Sent: 	Wednesday, April 24, 2002 8:49 AM
> To: 	histonet@pathology.swmed.edu
> Subject: 	EFFECTS OF DECALCIFICATION OF BONE ON IMMUNO
> 
> Good Morning all, happy spring....
> Anyone know of any articles published concerning the effects of
> decalcification on antigenicity of tissue used for immuno?
> I am running Ki67 (mib-1) on osteosarcomas and chondromas and there seems
> to
> be a loss of positive staining on most, but not all of them. I suspect
> that
> the decalcification process has effected them. 
> thanks
> Jim Fessler
> Pennsylvania Hospital
> Philadelphia, Pa
> 
> 
>