RE: EFFECTS OF DECALCIFICATION OF BONE ON IMMUNO
There was a great article in BioTechniques, Vol. 32, No.2 (2002) 296-298
about decal & Zebra Fish & Immunos.
If you can't access the article give me a shout with your Fax and I'll fax
it to you.
Center for Cardiovascular Research
University of Rochester Medical Center
KMRB Room 2-9816
601 Elmwood Avenue.
PO Box 679
Rochester, New York 14642
> From: Fessler, Jim
> Sent: Wednesday, April 24, 2002 8:49 AM
> To: email@example.com
> Subject: EFFECTS OF DECALCIFICATION OF BONE ON IMMUNO
> Good Morning all, happy spring....
> Anyone know of any articles published concerning the effects of
> decalcification on antigenicity of tissue used for immuno?
> I am running Ki67 (mib-1) on osteosarcomas and chondromas and there seems
> be a loss of positive staining on most, but not all of them. I suspect
> the decalcification process has effected them.
> Jim Fessler
> Pennsylvania Hospital
> Philadelphia, Pa
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