Need help for decal samples

From:Amy Porter

We are working on a research project involving mouse bone.  Trying to perform Luna's stain for eosinophils.  Tissues have been decaled with formic for a predetermined length of time by the researcher, then rinsed in running water for 4 hours and processed by standard methods.  The stain seems to be diffusely staining no matter the variations that we try, without much luck seeing the eosinophils.  All samples that are formalin fixed without decal are staining beautifully.  Any suggestions would be helpful.

Amy S.Porter, HT(ASCP)
Michigan State University
College of Human Medicine

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