Microwave Decalcification


I have been looking at the effects of decalcifying rodent feumr/knee joints
in the microwave with formic acid and have found that the process has left
the marrow looking somewhat "muddy" after staining with H&E.  The nuclei are
not crisp at all, and I have found that the effect worsens the longer the
bones remain in the microwave.  Any suggestions?  Should I try a different
decalcifying agent?  Or does this effect accompany all specimens decalcified
in the microwave?  Thanks in advance.

Kevin Jackson
Aventis Pharmaceuticals
Associate Scientist
Anatomic Patholgy (US)
(908) 231-2986

<< Previous Message | Next Message >>