Microwave Decalcification
From: | Kevin.Jackson@aventis.com |
I have been looking at the effects of decalcifying rodent feumr/knee joints
in the microwave with formic acid and have found that the process has left
the marrow looking somewhat "muddy" after staining with H&E. The nuclei are
not crisp at all, and I have found that the effect worsens the longer the
bones remain in the microwave. Any suggestions? Should I try a different
decalcifying agent? Or does this effect accompany all specimens decalcified
in the microwave? Thanks in advance.
Kevin Jackson
Aventis Pharmaceuticals
Associate Scientist
Anatomic Patholgy (US)
(908) 231-2986
kevin.jackson@aventis.com
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