DAB+nickel no primary problem

From:Donna McCleary

Hello histonet!

I am currently using an ABC immunostain protocol that incorporates DAB and nickel for visualization.  Unfortunately, I sometimes get 
intense staining of my no primary controls.  In the past, making up a fresh batch of TBS solved the problem, however today the fresh TBS 
still turned my no primary controls (and all the other tissue as well) into charcoal.  Has anyone else encountered this and what was your 

Donna McCleary
Graduate Assistant
Pharmacology & Therapeutics
University of South Florida
College of Medicine

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