Cryostat sectioning and RNase

From:Bebhinn Treanor

Hello,

I am new to this area of work and am starting an ISH experiment on monkey 
brain tissue.  I am hoping someone can provide a few suggestions on how to 
avoid RNA degradation during cryostat sectioning.  What precautions should 
I be taking with regard to the blade and brushes, or the guide plate?  Do 
you recommend wearing a face mask?  Any suggestions would be greatly 
appreciated.

Thanks,
Bebhinn

Bebhinn Treanor
Research Technician
Department of Anatomy and Developmental Biology
University College London
Gower Street, London
WC1E 6BT
phone: 020 7679 3904
fax: 020 7679 7316
email: b.treanor@ucl.ac.uk





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