Cryostat sectioning and RNase
Hello,
I am new to this area of work and am starting an ISH experiment on monkey
brain tissue. I am hoping someone can provide a few suggestions on how to
avoid RNA degradation during cryostat sectioning. What precautions should
I be taking with regard to the blade and brushes, or the guide plate? Do
you recommend wearing a face mask? Any suggestions would be greatly
appreciated.
Thanks,
Bebhinn
Bebhinn Treanor
Research Technician
Department of Anatomy and Developmental Biology
University College London
Gower Street, London
WC1E 6BT
phone: 020 7679 3904
fax: 020 7679 7316
email: b.treanor@ucl.ac.uk
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