Brazil's fluid


Here's a message Tony is having trouble posting to the list:

Hi all,

My laboratory has used a fixative called BRAZIL'S FLUID for fixing tissues
where small amounts of glycogen are being sought. It is supposedly a good
fixative for glycogen. Unfortunately I have no reference for this fixative
nor its uses. The formula is as follows:

Absolute alcohol 	1650ml
Formalin 	600ml
Trichloracetic acid 	7.5g
Picric acid 	10g
Dissolve picric acid, then Trichloracetic acid in the alcohol, then add the

Fixation time:- 
Normal block size 3-4 mm thick 	24 hours
Needle biopsy 	3-4 hours

The only one I know of is Dubosq-Brasil fluid which is similar (an alcoholic
Bouins fluid):

Ethylalcohol 80%    150ml
picric acid         1g
immediatly before use add:
Formol 35%           60ml
acetic acid, pure    15ml.

From: Bock, P.: "Romeis Mikroskopische Technik", 17th. ed., p. 98.

Would anyone have a reference for BRAZIL'S FLUID that they could share with

Thanks muchly,

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318 


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