Brazil's fluid
Here's a message Tony is having trouble posting to the list:
Hi all,
My laboratory has used a fixative called BRAZIL'S FLUID for fixing tissues
where small amounts of glycogen are being sought. It is supposedly a good
fixative for glycogen. Unfortunately I have no reference for this fixative
nor its uses. The formula is as follows:
Absolute alcohol 1650ml
Formalin 600ml
Trichloracetic acid 7.5g
Picric acid 10g
Dissolve picric acid, then Trichloracetic acid in the alcohol, then add the
formalin.
Fixation time:-
Normal block size 3-4 mm thick 24 hours
Needle biopsy 3-4 hours
The only one I know of is Dubosq-Brasil fluid which is similar (an alcoholic
Bouins fluid):
Ethylalcohol 80% 150ml
picric acid 1g
immediatly before use add:
Formol 35% 60ml
acetic acid, pure 15ml.
From: Bock, P.: "Romeis Mikroskopische Technik", 17th. ed., p. 98.
Would anyone have a reference for BRAZIL'S FLUID that they could share with
me?
Thanks muchly,
Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318
http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html
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