Well... I am in the process of embedding and sectioning a
multi-layer tissue culture grown in a petri dish. However, I'm not using
paraffin. I'm using glycol methacrylate (Technovit 7100). I process the
plate through the VIP tissue processor up to the xylenes, remove and place
in increasing strengths of glycol and then embed in GMA, allow to
polymerize and section on the Polycut E. So far...so good! We tried this
on a blank petri dish and it worked just fine.
Your petri dish will not cut with paraffin embedding. For you to
do paraffin, you will need to remove the cells from the petri dish by
trypsinizing them and then centrifuge the solution, decant the supernatant,
process the cell button and hope for the best. Just a thought...you might
try processing the petri dish without the cells (pilot study) through the
processor and fill the petri dish (if it hasn't dissolved or distorted due
to the xylenes) with wax, allow to chill and pop it out of the petri dish
and see if the cells (use a substitute if you have limited cultures) attach
to the surface of the wax instead of remaining on the petri dish. Then you
could re-embed the trimmed paraffin circle into a metal
basemold. Remember, you are dealing with cells, not "hunks" of tissue.
Also, you can stain the cell in the dish with toluidine blue if
you just want a "quick and dirty" stain for viewing microscopically.
If you want my exact processing schedule for GMA, I will be glad
to send it to you.
Linda Jenkins, HT
Dept. of Bioengineering
Clemson, SC 29634-0905
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