Tetracycline fluorescence imaging

From:Dan Beniker

Hello all,

We are conducting some bone growth studies and are trying to visualize bone
double labelled with tetracycline and calcein. We're using a Nikon E400
microscope and currently are using a Nikon B-2A filter cube which excites
from 450-490 nm. This seems to be fine for calcein fluorescence, but we
aren't able to see the tetracycline label at all. The Nikon dealer
recommends a BV-2A filter for tetracycline fluorescence while the Nikon web
site recommends other filter cubes. Does anyone out there have experience
with this scope/filter/label combo? If so, would you be able to shed some


Dan Beniker
LifeCell Corp

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