Re: whole organ fixation with color retention
From: | =?iso-8859-1?q?Tony=20Henwood?= |
Louri,
My suggestion is to slice the organs, fix in buffered
formalin or Wentworth's fixative (see most
histotechnology textbooks for the formula) for 4 weeks
or more. Mount specimen in a perspex museum box. Rinse
specimen in 95% ethanol and leave in ethanol untill
colour comes back (brown bloody areas appear red),
replace ethanol with light paraffin oil, seal the box,
label and all done.
Tony
--- Louri Caldwell wrote: > Hi
everyone,
>
> We have a researcher interested in retaining whole
> organs for teaching
> purposes, but would like the original color of the
> organ to remain intact.
> I know a similar topic was addressed not too long
> ago, and I have read the
> archives, but wanted to see if anyone had
> suggestions other than
> plastination. Ideally, he would like a fixative -
> and the only one I've
> found a reference for contains chloral hydrate -
> which he can't purchase as
> a DEA number is required to do so.
>
> Any suggestions you may have would be greatly
> appreciated.
>
> Thank you,
> Louri
>
>
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=====
Tony Henwood JP BAppSc GradDipSysAnaly CT(ASC)
Laboratory Manager
Histopathology Department
The Childrens Hospital at Westmead
Locked Bag 4001 WESTMEAD, 2145 Australia. http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html
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