Re: dried material
I would place the tissue in Ruffers solution:
95% ethanol 300ml
2% formalin 500ml
5% Sodium carbonate 200ml
Leave for 24-48 hours.
Process as normal, preferably on a gentle process (low
heat, no vacuum, shorter times)
This should do the trick.
--- Tora Bardal wrote: >
> I have been asked to examine dried salted/unsalted
> cod sections with LM.
> I would like to have some ideas from you before I
> start. (Not Bacalao
> recipes:-) )
> Fixation?.............and what happens with the
> dried muscle in the solution?
> Embedding? Paraffin ok?
> Historesin (or similar) or Epon?
> Tora Bardal Chief Engineer
> Department of Zoology,
> Norwegian University of Science and Technology
> Brattoera Research Center
> N-7491 Trondheim
> Norway tlf: + (47)73 59 09 38
> E-mail: Tora.Bardal@nt.ntnu.no fax:+ (47)73 59 63
Tony Henwood JP BAppSc GradDipSysAnaly CT(ASC)
The Childrens Hospital at Westmead
Locked Bag 4001 WESTMEAD, 2145 Australia. http://www.histosearch.com/homepages/TonyHenwood/default.html
http://www.sold.com.au - SOLD.com.au Auctions
- 1,000s of Bargains!
<< Previous Message | Next Message >>