Re: dried material

From:Gayle Callis

You should rehydrate the dried tissue in Ruffer's solution 50 parts water,
30 parts absolute alcohol, 20 parts 5% sodium carbonate (or 0.6 g sdoum
carbonate in 42 ml water, add 18 nl absolute ethanol), rehydrate 8 hours or
longer depending on size of tissue, the proceed to processing alochols.
The alcohol helps fix the tissue, 

This came from a paleopathology laboratory, M Zimmerman who also published
the technic in Laboratory Medicine 1976.

Gayle Callis
Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367
406 994-4303 (FAX)

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