Re: control tissue for TUNEL
I've had good luck with TUNEL on mouse intestine. Some parts seem to show more + cells than others (jejunum and duodenum more than colon). The staining is generally where it should
be, in isolated cells at the luminal surface of the tissue at the ends of villi, and it correlates with the morphology: shrunken away from the surrounding tissue, condensed chromatin.
But not always! TUNEL is a frustrating stain. Have also tried skin (nothing) and ovaries (+). Going to try cleaved caspase-3 now. Somebody here got staining against full-length
caspase-3 of a few cells among many healthy cells but I don't understand that: shouldn't it stain the healthy cells too? (The antibody recognizes cleaved as well as full-length).
Greg Dobbin wrote:
> Hi Teri,
> Teste is a good one. Sperm cells are continually produced and
> "dying"! It works well for us.
> Date sent: Thu, 14 Mar 2002 08:54:02 -0600
> From: "Johnson, Teri"
> Subject: control tissue for TUNEL
> To: "Histonet (E-mail)"
> > I'm getting ready to start working up the markers for apoptotic cells (TUNEL and caspase-3) in mouse tissue. I'm wondering what tissue is best to use as a control. Skin, perhaps?
> > Thanks!
> > Teri Johnson
> > Managing Director Histology Core Facility
> > Stowers Institute for Medical Research
> > 1000 E. 50th St.
> > Kansas City, Missouri 64110
> > email@example.com
> ------- End of forwarded message -------
> Greg Dobbin
> Pathology Lab
> Atlantic Veterinary College, U.P.E.I.
> 550 University Ave.
> Charlottetown, P.E.I.
> Canada, C1A 4P3
> Phone: (902)566-0744
> Fax: (902)566-0851
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