Re: collagen type I

From:rueggp
Harry,
The University of Iowa Hybridoma Bank sells an excellent antibody to collagen I, unfortunately it is a monoclonal and would not be a good choice for mouse tissue.  I have used it on human and rat tissue successfully with pepsin digestion with zinc formalin fixation.  I use the supernate, neet for one hour with DAKO's LSAB or Envision detection system.  You might try PharminGen for an anti-mouse collagen antibody.

Harry_Sweigard@biogen.com wrote:

 
Hello all,

I have a question for those who have a good IHC method for detecting collagen type I in human and mouse tissue.  We've had great success with Novocastra collagen I primary and citrate boiling in the past.  Unfortunately we've been informed that Novocastra has discontinued their collagen I primary.  I've tried Chemicon and RDI using various digestions but they don't quite live up to the quality that we were getting with the Novocastra antibody.  Ideally we'd like to detect in mouse and human.  If anyone has antibody/digestion recommendations for either I'd very much appreciate their advice.  Thanks.

-Harry Sweigard

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