Re: Inconsistent ER results


We find that tissue will lose its antigenicity for ER in just a matter of 
days.  We cut our slides for ER, including the positive control tissue, fresh 
each time, and stain them the same day.  It also loses strength if allowed to 
go through a dezenkerization procedure (Iodine followed by Hypo).  This may 
be why you are getting a decrease in sensitivity and an increased variability 
of results.

Ellen Yee

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