From: | Chewy71874@aol.com |
Tom, We find that tissue will lose its antigenicity for ER in just a matter of days. We cut our slides for ER, including the positive control tissue, fresh each time, and stain them the same day. It also loses strength if allowed to go through a dezenkerization procedure (Iodine followed by Hypo). This may be why you are getting a decrease in sensitivity and an increased variability of results. Ellen Yee Sacramento