Re: How long are subbed slides good?

From:rueggp

i thought things in the water bath like (Stay ON, if it goes in the WB) negated
the charge on subbed slides????
patsy

Melissa Jensen wrote:

> Maybe using gelatin in your bath water is the problem.I worked in a histo
> lab prior to the one I am at.We only used gelatin there..Had trouble keeping
> tissue on slides during gms procedure etc.I was introduced to Stay on at my
> current facility.It is great! Nothing falls off..no curling,You don't even
> have to let slides cool before putting them in the first xylene....There is
> another product called Halt.We tried it..but it reduced the surface tension
> of the water so much..All tissue no matter what curled...didn't adhere to
> the slide......We use subbed slides..they all stay in tact..
> Hope this helps!
> ----- Original Message -----
> From: "Cathy Gorrie" 
> To: 
> Sent: Thursday, April 11, 2002 7:43 PM
> Subject: Re: How long are subbed slides good?
>
> > We regularly cut 30-40 Ám rat brain sections, place on subbed slides
> > (0.8% gelatin with 0.05% chromium potassium sulphate) and air dry
> > overnight before staining. Normally this is absolutely fine,and the
> > age of subbed slides has varied from a few weeks to very old.
> >
> > Recently however, we have been having trouble with the same problems
> > you are experiencing, BUT only on one series and one type of
> > staining, rather than just any liquid. ie sections have been cut and
> > placed on slides in series of two, one stays on through the stain
> > over 4 days but the Cresyl Violet (10 mins) stained sections are
> > lifting. I don't think it is the age of the subbing therefore, but
> > possibly is due to the quality of subbing or staining solutions.
> > There was some discussion on this list a while ago about alkaline
> > solutions causing gelatin to become more soluble in water, hence the
> > addition of chrome alum etc etc, and I suspect this is the root of
> > the problem. Maybe one batch of subbing solution was not made
> > correctly, or the a batch of staining solution is slightly more
> > alkaline than usual.
> >
> > Hope this gives you a starting point.
> >
> > Cathy
> >
> >
> > At 2:37 PM -0400 10/4/02, Knight, Brandon wrote:
> > >I've been having a problem with 40um mouse brain sections coming off of
> > >subbed slides when placed in any liquid (for instance, when going through
> > >the staining process).  The edges may fold over, they may wrinkle, or
> slide
> > >off completely.  They were sufficiently dried (some for a couple of
> weeks).
> > >The slides that I'm using were subbed in August of 2000.  Is it possible
> > >that the gelatin has degraded and that these slides are no longer subbed,
> or
> > >could there be another explanation?
> > >
> > >Brandon Knight
> > >Children's Research Institute
> > >Center for Genetic Medicine
> > >Children's National Medical Center
> > >Washington, D.C.
> >
> > --
> > ---------------------------------------------------
> > Cathy Gorrie
> > Scientific Officer
> > Neural Injury Research Unit,
> > School of Medical Sciences,
> > University of New South Wales
> > Sydney, N.S.W. 2052
> >
> > Phone: 61-2-9385 2462
> > Fax   : 61-2-9313 6252
> > e-mail: c.gorrie@unsw.edu.au
> >
> >






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