Try air drying the brain sections first. I wait until the sections are
completely air dried before putting in the oven. Maybe the same thing
would have happened had the slides been freshly subbed. Actually, I
don't know how long they're good for. But your combination of animal
tissue plus it being brain tissue plus it being thick is I guess too
much against the odds of it staying on. Just my 2 cents worth....


Salvacion S. Delovino