Re: Hale's method for colloidal iron

From:Bryan Llewellyn

According to Pearse's Histochemistry: Theoretical and applied, Hales
colloidal iron is as follows:

Fix in Wolman, Carnoy or formalin.  Paraffin sections.  Avoid adhesives.

1.    Sections to water.
2.    Mix 1 vol dialysed iron and 1 vol 2M acetic acid.  Apply for 10 mins.
3.    Wash very well with distilled water to remove all traces of iron.
4.    Do Perls' prussian blue.
5.    Wash well with water.
6.    Counterstain with neutral red 1 min.
7.    Dehydrate, clear, mount

Acid mucins - blue
Nuclei - red

Rinehart and Abu'l Haj dialysed iron
75 g ferric chloride
250 mL water
Dissolve and add 100 mL glycerol.
While stirring, add 55 mL strong ammonia.
Dialyse against distilled water for three days, changing the water
This mixture triples in volume by the end of dialysis.

----- Original Message -----
From: "Goodwin, Diana" 
To: "'Histonet'" 
Sent: Thursday, April 04, 2002 8:16 AM
Subject: Hale's method for colloidal iron

> Greetings, Netters.
> Does anyone have a copy of Hale's method for colloidal iron?  My
> has asked for it specifically, probably because it is cited in the
> literature for differentiation of chromophobe RCC.  The only method I have
> on hand is the Muller-Mowry method in Frieda's book.  Frieda, if you're
> there, can you tell me what the difference is?
> Thanks in advance,
> Diana Goodwin,  HT
> Trenton,  NJ

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