Re: Hale's Colloidal Iron Technique, Culling; 3rd edition
There is a newer copy of this book:
Cellular Pathology Technique, 4th Edition, Culling, Allison and Barr, 1985,
ISBN 0 407 72903 8
Sultan Qaboos University,
----- Original Message -----
From: "Victoria Baker"
To: "HistoNet Server"
Sent: Friday, April 05, 2002 7:05 PM
Subject: Hale's Colloidal Iron Technique, Culling; 3rd edition
> "Hale's technique is based upon the affinity of acid
> groups for colloidal iron at a low pH. The iron forms
> a chelate with the acid groups and may then be
> demonstrated by the Prussian Blue reaction.
> While this method is excellent for the demonstration
> of acid mucins it is inferior to Alcian blue as a
> specific method for histochemical investigation.
> Special solutions required
> Dialysed iron .... 1 volume
> 2M acetic acid.....1 volume
> Dialysed Iron Solution (Reinhardt and Abu'l Haj)
> Dissolve gradually 75gm ferric chloride in 250ml
> distilled water, adding 100ml glycerol, followed by
> the gradual addition of 55ml of 28% ammonia with
> constant stirring. This mixture is dialysed against
> regularly changed distilled water for 3 days.
> Acid-ferrocyanide Solution
> Equal parts of 2% potassium ferrocyanide and 2%HCl.
> 1. Bring sections to water.
> 2. Flood with dialysed iron solution (either solution
> works well)for 10 minutes.
> 3. Wash well with distilled water.
> 4. Flood with acid ferrocyanide solution for 10
> 5. Wash well in water.
> 6. Counterstain lightly with 0.1% neutral red or
> 7. Rinse in water, and dehydrate rapidly.
> 8. Clear in xylol and mount in D.P.X. or H.S.R.
> Acid mucopolysaccharides.....Bright blue
> Other tissue constituents....Shades of red."
> The exact script (minus any typos on my behalf) comes
> Handbook of Histopathological and Histochemical
> Techniques; 3rd edition, 1974. C.F.A. Culling.
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