There is a newer copy of this book:

Cellular Pathology Technique, 4th Edition, Culling, Allison and Barr, 1985,
ISBN 0 407 72903 8

Evelyn Kaplan
Sultan Qaboos University,
Oman


----- Original Message -----
From: "Victoria Baker" 
To: "HistoNet Server" 
Sent: Friday, April 05, 2002 7:05 PM
Subject: Hale's Colloidal Iron Technique, Culling; 3rd edition


> "Hale's technique is based upon the affinity of acid
> groups for colloidal iron at a low pH.  The iron forms
> a chelate with the acid groups and may then be
> demonstrated by the Prussian Blue reaction.
> While this method is excellent for the demonstration
> of acid mucins it is inferior to Alcian blue as a
> specific method for histochemical investigation.
>
> Special solutions required
> Dialysed iron .... 1 volume
> 2M acetic acid.....1 volume
>
> Dialysed Iron Solution (Reinhardt and Abu'l Haj)
> Dissolve gradually 75gm ferric chloride in 250ml
> distilled water, adding 100ml glycerol, followed by
> the gradual addition of 55ml of 28% ammonia with
> constant stirring.  This mixture is dialysed against
> regularly changed distilled water for 3 days.
>
> Acid-ferrocyanide Solution
> Equal parts of 2% potassium ferrocyanide and 2%HCl.
>
> Technique
> 1.  Bring sections to water.
> 2.  Flood with dialysed iron solution (either solution
> works well)for 10 minutes.
> 3.  Wash well with distilled water.
> 4.  Flood with acid ferrocyanide solution for 10
> minutes.
> 5.  Wash well in water.
> 6.  Counterstain lightly with 0.1% neutral red or
> safranin.
> 7.  Rinse in water, and dehydrate rapidly.
> 8.  Clear in xylol and mount in D.P.X. or H.S.R.
> resin.
>
> Results
>
> Acid mucopolysaccharides.....Bright blue
> Other tissue constituents....Shades of red."
>
>
> The exact script (minus any typos on my behalf) comes
> from:
>
> Handbook of Histopathological and Histochemical
> Techniques;  3rd edition, 1974.  C.F.A. Culling.
>
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