RE: glucose reference
Just cite the solubility, 1g in 1 ml water, from the Merck Index, p. 699 of
the 11th edition (Merck & Co., Rahway, NJ, 1989). It should be obvious from
that that glucose washes out in aqueous media. Fixing in ethanol doesn't
help: 1 ml ethanol dissolves 17 mg glucose, much more than one would find in
any human tissue.
Allen A. Smith, Ph.D.
School of Graduate Medical Sciences
Podiatric Medicine and Surgery
Miami Shores, Florida 33161-6695
From: Haines, Ashley N. [mailto:ANH001@dental.umaryland.edu]
Sent: Monday, April 08, 2002 4:57 PM
To: Histonet (E-mail)
Subject: glucose reference
I know that glucose is highly soluble and is typically washed out of tissue
sections, unless it is being stored in the form of glycogen which is well
preserved. However, I can't seem to find a reference that states this.
Specifically: that glucose is highly soluble for the purposes of histology
and therefore difficult to visualize via staining. I've checked the 5
different histology reference books I have here in the lab. If anybody can
help I'd greatly appreciate it... Thanks!
Ashley N. Haines
Dept. of OCBS
Univ. of Md. Dental School
666 W. Baltimore St.
Baltimore, MD 21201
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