RE: Trouble Shooting
We had a similar problem a few years ago. You didn't mention what you did
with the tissue. In our case, we reverse processed by hand and then
reprocessed(skipping fixation) on the processor. The sections were by no
means beautiful(overprocessed) but they were diagnosable.
From: DeLovino, Salvacion S. [mailto:Salvacion.DeLovino@cshs.org]
Sent: Wednesday, April 10, 2002 11:23 AM
To: 'HistoNet Server'
Subject: Trouble Shooting
We had a problem with our tissue processing last week and until now,
they are still arguing about what should and shouldn't have been done. What
happened was, our MLA used methyl alcohol instead of xylene when she changed
the solutions. So it was methyl alcohol instead of xylene right before
paraffin. It was her fault for not reading the label but couldn't really
blame her as the containers look alike. Anyways, we didn't know at first
that that was what happened but we noticed the tissues were hard and brittle
on the edges but soft in the middle. Was there something we could have done
to save the tissues? Please help us settle the arguments!
Thanks a lot in advance.
Salvacion S. Delovino
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