I would have put the tissue back in xylene to remove the paraffin, then
absolute alcohol, xylene and paraffin again. the tissue would have been a
little crisp, but salvageable. 

Joyce Weems
Pathology Manager
Saint Joseph's Hospital of Atlanta


	-----Original Message-----
	From:	DeLovino, Salvacion S. [SMTP:Salvacion.DeLovino@cshs.org]
	Sent:	Wednesday, April 10, 2002 2:23 PM
	To:	'HistoNet Server'
	Subject:	Trouble Shooting


		Hello Histonetters,

		We had a problem with our tissue processing last week and
until now,
	they are still arguing about what should and shouldn't have been
done. What
	happened was, our MLA used methyl alcohol instead of xylene when she
changed
	the solutions. So it was methyl alcohol instead of xylene right
before
	paraffin. It was her fault for not reading the label but couldn't
really
	blame her as the containers look alike. Anyways, we didn't know at
first
	that that was what happened but we noticed the tissues were hard and
brittle
	on the edges but soft in the middle. Was there something we could
have done
	to save the tissues? Please help us settle the arguments!
		Thanks a lot in advance.

		Salvacion S. Delovino