RE: Tetracycline fluorescence imaging

From:Dan Beniker

Thanks for the respnse, Ashley,

I was on that site earlier and noticed that the dealer recommended one (the
BV-2A.) doesn't list tetracycline as one of the recommended chromaphores. In
addition, it looks like the excitation wavelength for tetracycline (390 nm)
falls just outside of the specs for the BV-2A (400-440nm). The dealer didn't
seem to think that would be a problem, but I wanted to get some input from
some folks who have used that cube, just to be sure....

> -----Original Message-----
> From:	Haines, Ashley N. []
> Sent:	Wednesday, April 10, 2002 3:49 PM
> To:	'Dan Beniker'
> Subject:	RE: Tetracycline fluorescence imaging
> Dan,
>  You need ultraviolet light to visualize the tetracycline.  So, you need
> to
> get down closer to 400nm for excitation, which is what the BV-2A will do.
> That probably means visualizing your two labels separately...
> Ashley
> -----Original Message-----
> From: Dan Beniker []
> Sent: Wednesday, April 10, 2002 2:55 PM
> To: 'Histonet'
> Subject: Tetracycline fluorescence imaging
> Hello all,
> We are conducting some bone growth studies and are trying to visualize
> bone
> double labelled with tetracycline and calcein. We're using a Nikon E400
> microscope and currently are using a Nikon B-2A filter cube which excites
> from 450-490 nm. This seems to be fine for calcein fluorescence, but we
> aren't able to see the tetracycline label at all. The Nikon dealer
> recommends a BV-2A filter for tetracycline fluorescence while the Nikon
> web
> site recommends other filter cubes. Does anyone out there have experience
> with this scope/filter/label combo? If so, would you be able to shed some
> light?
> Thanks,
> Dan Beniker
> LifeCell Corp

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