RE: Cox-1 and Cox-2

From:"Connolly, Brett M"

Dana,
I would recommend Oxford Biomedical's antibodies. They also supply the
matched preimmune. Try citrate antigen retrieval and overnight incubation in
the 1:2000-1:4000 range.
Brett

Brett M. Connolly, Ph.D.
Merck Research Laboratories
Dept. of Neuroscience
WP26A-3000
PO Box 4
West Point, PA 19486
PH 215-652-2501
fax. 215-652-2075
e-mail. brett_connolly@merck.com


-----Original Message-----
From: Dana Settembre [mailto:settembr@umdnj.edu]
Sent: Thursday, April 11, 2002 3:37 PM
To: histonet@pathology.swmed.edu; settembr@UMDNJ.EDU
Subject: Cox-1 and Cox-2


Hello,
I'd like to run Cox-1 and Cox-2 on FFPE human tissue.  Can't seem to get it
to work.
Which vendor do you use?  What about control? Pretreatment?  Dilution?
Help!

Thank you,
Dana Settembre
University Hospital-UMDNJ
Newark, NJ




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