LFB on frozen brain tissue

From:Stephanie Rodriguez

Hi Mikhail,
I don't know if you've had any other responses yet; I've been out of the lab for a few days, and just saw your message.  I routinely stain fresh frozen mouse brains with Luxol Fast Blue and Cresyl Violet counterstain.  I do fix them in formalin for about 5 minutes first.  We also de-fat them in chloroform for 5 minutes, but I don't know that it's necessary-we just do it because we prep large quantities of slides at the same time, and some are used for in-situ hybridization.  Our stain comes out beautifully with this method.
Good luck!
Stephanie Rodriguez 
Primal, Inc.
Seattle, WA

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