Antibody Characterization

From:"Patterson, Noelle (NIDDK)"


Anyone out there been tasked to characterize the specificity of a novel
antibody using IHC as "proof"?

  If so, what approaches did you use?  How did discern "real" from
background?  Particularly since I am to characterize an antibody to a
protein that is not (at all) understood; and has no "expected" pattern of
expression.  I thought about all the standard background blocking
techniques, but feel I need to get more "sophisticated" to convince

I welcome any helpful journal references.  I have a few, but am always
looking for "better ways" to do things.

Noelle Patterson
Bethesda, MD   20889-5603 

<< Previous Message | Next Message >>