wrinkles in brain tissue(paraffin embedded)
Dear histonetters,
I have been having a problem with wrinkles in mice brain(approximately 30 days
old). The tissue don't look wrinkled when I cut it, but when I lay the tissue
in the water bath, it wrinkles all along the edges. I have changed angles on
microtome, changed disposable blades several times, even changed water bath
(tried with & without gelatin). Should I change my water bath temp? Right now
I have it at 42 degree Centigrade. Several days ago, I cut mice brain without
any problems.
Any help is greatly appreciated.
Thank you,
Dina
dgaupp@tulane.edu
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