silver stain

From:"R.J. Rushmore" <>

Hello Histonetters,

I am trying to use a varient of the Fink/Heimer degeneration technique used
by Wiitanen (1969).  It uses potassium permaganate (0.05%), oxalic
acid/hydroquinone, uranyl nitrate (5%), silver nitrate (0.3%) followed by
ammoniacal silver solution and nauta reducer.

My attempts thus far have failed.  Is there anyone who can give advice on
the wizardry of silver staining, or who has used the Wiitanen myelin
degeneration technique and has perhaps a more complete protocol than the one
in the 1969 Brain Research Paper?

Thanks very much,

Jarrett Rushmore
Boston University School of Medicine

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