From:Cathy Mayton <Cathy@wasatchhisto.com>

Dear Renee,

I have tried the Tetrachrome but another alternative is a stain sold by
Surgipath, called the Sanderson's Rapid Bone Stain.  The stain is completed
in approximately 5 minutes or less.  It was developed to stain ground
sections of mineralized bone.  It not only differentiates osteoid from
mineralized bone but also viable bone from dead bone.  There are 2
counterstains that can be used; acidified acid fuchsin or van Gieson's.
The osteiod is very obvious and the stain is extremely easy to do.

Hope this helps,

GLP Compliant Laboratory

Cathy A. Mayton
Project Director
Wasatch Histo Consultants, Inc.

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