frozen eyes - my challenge for the week

From:Andrea Grantham <algranth@u.arizona.edu>


I have just had a call from a resident who wants to get frozen sections on 
rabbit eyes. The purpose of frozens over paraffin sections is that a set of 
the eyes are injected with Indocyanine Green dye and another will be 
injected with 1% Trypan Blue dye. He is interested in the anterior chamber 
of the eye - specifically the lens and is looking for presence of and 
penetration of the dye into the lens.
I can find no procedure in any of my books for freezing eyes for frozen 
sections. Help!
Does anyone out in histoland have a protocol for freezing eyes, 
tips/suggestions for sectioning frozen eyes or maybe know the source of a 
good reference for doing this? I'd be eternally grateful!
Andi Grantham
.....................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
: (office:  AHSC 4212)          P.O. Box 245044                     :
: (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
: (FAX:  520-626-2097)          (email:  algranth@u.arizona.edu)       :
:...................................................................:
           http://www.cba.arizona.edu/histology-lab.html




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