frozen/unfixed tissue

Dear Histonetters,

    Has anyone heard of performing H&E stain on tissue that has been frozen/not 
fixed at -80 degrees Centrigrade for about 2 weeks?  How would you go about 
staining for BEST RESULTS?  My guess is that you would just do frozens. Oh by 
the way, the tissue is kidney from a baby rat. I need to cut through the entire 
tissue and perform H&E.  I do know that H&E's come out better once they are 
fixed,processed, and embedded in paraffin. I would rather cut tissue that has 
been fixed and paraffin embedded(the results come out so much better).

Any help is greatly appreciated.



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