Has anyone heard of performing H&E stain on tissue that has been frozen/not
fixed at -80 degrees Centrigrade for about 2 weeks? How would you go about
staining for BEST RESULTS? My guess is that you would just do frozens. Oh by
the way, the tissue is kidney from a baby rat. I need to cut through the entire
tissue and perform H&E. I do know that H&E's come out better once they are
fixed,processed, and embedded in paraffin. I would rather cut tissue that has
been fixed and paraffin embedded(the results come out so much better).
Any help is greatly appreciated.
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