Re: help with PFA fixation
|From:||Sunil Thomas K <email@example.com>|
Thanks for mailing your opinion. Should try out a
different PFA powder to see if it gives a better
result. The fixation is better now. Probably the
How long should one perfuse with saline/PBS before
switching over to PFA?
I switch over after about 5 minutes when the
efflux is weakly blood stained (for a 20-30 gm
How slow should be the flow rate?
I guess it should be roughly equal to
Your thoughts regarding these questions will be most
Sunil Thomas K
--- Natalie Prigozhina <firstname.lastname@example.org> wrote:
> Dear Dr. Thomas:
> Your problem may be in using old PFA powder. I don't
> think increasing the
> concentration would help. Try some fresh PFA (at
> least borrow from somebody
> a little to check if it gives you better results).
> Also depending on the
> structures you are staining, you may be able to use
> simple methanol
> fixation (5-10 min in -20C methanol). I am a cell
> biologist and work with
> cells cultures. Hope it helps with your tissues.
> Dr. Natalie Prigozhina
> The Scripps Research Institute,
> La Jolla, California
> P.S. Next time you post in a newsgroup, consider
> addressing to people as
> "Dear Sir or Madam".
> Good luck with your stainings.
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