Dina,
You do have an option to fix and process this tissue to paraffin, if there is no
reason to be working with frozen sections. Morphology will be better in well fixed
and processed tissue. Just put your frozen tissue in neutral buffered formalin, let
it come to room temperature, trim if needed, fix well and process to paraffin.
Katri

Katri Tuomala
Anatomic Pathology
St.Joseph's Healthcare
Hamilton, Ontario, Canada

dgaupp@tulane.edu wrote:

> Dear Histonetters,
>
>     Has anyone heard of performing H&E stain on tissue that has been frozen/not
> fixed at -80 degrees Centrigrade for about 2 weeks?  How would you go about
> staining for BEST RESULTS?  My guess is that you would just do frozens. Oh by
> the way, the tissue is kidney from a baby rat. I need to cut through the entire
> tissue and perform H&E.  I do know that H&E's come out better once they are
> fixed,processed, and embedded in paraffin. I would rather cut tissue that has
> been fixed and paraffin embedded(the results come out so much better).
>
> Any help is greatly appreciated.
>
> Thanks,
>
> Dina
> email: dgaupp@tulane.edu