Re: frozen/unfixed tissue

From:Barry Rittman <>

we have performed H and Es on tissue that hs been frozen in liquid nitrogen since
1975 and on tissue frozen at -80C for 15 years. There should be no problems with
your tissue providing that you have taken adequate precautions to prevent tissue
drying. If you have stored for two weeks in a plastic bag at -80C assume that
tissue is fine.
Barry wrote:

> Dear Histonetters,
>     Has anyone heard of performing H&E stain on tissue that has been frozen/not
> fixed at -80 degrees Centrigrade for about 2 weeks?  How would you go about
> staining for BEST RESULTS?  My guess is that you would just do frozens. Oh by
> the way, the tissue is kidney from a baby rat. I need to cut through the entire
> tissue and perform H&E.  I do know that H&E's come out better once they are
> fixed,processed, and embedded in paraffin. I would rather cut tissue that has
> been fixed and paraffin embedded(the results come out so much better).
> Any help is greatly appreciated.
> Thanks,
> Dina
> email:

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