Re: bx turn around times
|From:||Gayle Callis <email@example.com>|
Our EM lab makes sure the grids with sections are air dried well before
staining, overnight and up to 2 days. Ted Pella copper grids, 150 mesh,
cleaned with nitric acid etching before picking up the section.
At 09:45 AM 4/26/01 -0500, you wrote:
>>Do you have problems keeping sections on grids? I have had problems with the
>>200 mesh thin bar grids sections just fall off during staining.
>John, I use 300 copper mesh grids. If I TEM stain immediately after picking
>up ultrathins, yes all sections wash off. But placing copper grids in the
>40oC oven, seems to help adhere sections. Teresa
Veterinary Molecular Biology
Montana State University - Bozeman
Bozeman MT 59717-3610
404 994-4303 (FAX)
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