>i meant EM scope. i just looked your paper. using spurrs will certainly
> move
>things along. that is a very rapid protocol.  do you have problems with
>spurr
>being brittle? also i was wondering for EM do you mount on grids or formvar
>coated slot grids? we have to do the slot grid because the pathologist like
>to shoot a low mag 250X of each glomerulous.
>john hoffpauir

John, sorry I forgot to answer about grids. No I donot use formar coated
grids. For TEM 5 copper grids are picked up with ultrathin sections. Case
grids are placed in 40oC oven overnight. In am routine Reynolds TEM stain
is performed. Copper grids are ready for viewing. Teresa