Re: TRAP staining protocol for fixed decalcified bone sections

From:Gayle Callis <>

This was discussed on Histonet in last month,  and it may be worth your
time to check out the archives to see what people came up with IN ADDITION
your current answers received.  There were protocols and publications cited.

 At 08:38 PM 4/24/01 -0700, you wrote:
>Hello Everyone-
>	I have some formalin fixed decalcified bone sections and I would like to
>stain them for tartarate resistant acid phosphatase.  Does anyone out there
>know of a staining protocol, either there own or in the form of a
>reference, that has worked with fixed decalcified bone sections?
>Kristin Evans
Gayle Callis
Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
Bozeman MT 59717-3610

406 994-6367
404 994-4303 (FAX)

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