Re: Rat Tissue Processing
|From:||Gayle Callis <firstname.lastname@example.org>|
Try turning down temperature of waterbath.
What size brain tissues, 3 mm slabs or whole brain? and what processing
schedule do you use? More specifics will help with problem solving.
At 05:22 PM 4/20/01 -0400, you wrote:
>I wonder if any of you have a protocol that you wouldn't mind sharing for
>auto-processing rat CNS tissue for paraffin embedding. I am a novice at
>working with paraffin,using a Leica TP1050 and so far I have not found the
>right combination of steps to prevent the tissue from overly expanding and
>separating from the paraffin in the water bath.
>Any assistance would be greatly appreciated.
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Veterinary Molecular Biology
Montana State University - Bozeman
Bozeman MT 59717-3610
404 994-4303 (FAX)
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