Re: Rat Tissue Processing

From:Gayle Callis <>

Try turning down temperature of waterbath.  

What size brain tissues, 3 mm slabs or whole brain?  and what processing
schedule do you use?  More specifics will help with problem solving.  

At 05:22 PM 4/20/01 -0400, you wrote:
>Hello, All
>I wonder if any of you have a protocol that you wouldn't mind sharing for 
>auto-processing rat CNS tissue for paraffin embedding.  I am a novice at 
>working with paraffin,using a Leica TP1050 and so far I have not found the 
>right combination of steps to prevent the tissue from overly expanding and 
>separating from the paraffin in the water bath.
>Any assistance would be greatly appreciated.
>Get Your Private, Free E-mail from MSN Hotmail at
Gayle Callis
Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
Bozeman MT 59717-3610

406 994-6367
404 994-4303 (FAX)

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