Re: Rapid Processing of Prostate Bx's
From: | Gayle Callis <uvsgc@montana.edu> |
Our popcorn nuclei was the result of picking up a slide from waterbath,
then pressing it to a hotplate for more flattening of section. This was a
heat induced artifact, and we abandoned this practice. Went to a gentler,
lower heat for drying slides.
We use a small fine haired brush to apply marking ink to prevent plastering
the colored stuff onto tissues like frescos from the Sistine Chapel! It
will show up after processing.
At 12:03 PM 4/28/01 -0400, you wrote:
>Hello to all:
>Looking for some advice on our short run (2 hour processing) of prostate
>
>It looks like a bubble within the nuclei and stains lighter making it very
> These bx's are all well fixed (
> We have tried increasing the processing time to 4 hours-more
> Our
>overnight run also on occasion demonstrates this. We have ruled out the
> We use the same
> We also have a
>hazy or cloudy effect on these bx's even though we use the same staining
> Also, does anyone know of another type of colored
> We have
>used the Davidson(?) red, green and yellow, but find it takes to long to
dry
> Is there something to set this like bouins
> All help is appreciated.
>Tina Cahak
>Sacred Heart Hospital
>Eau Claire, WI
Gayle Callis
Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
Bozeman MT 59717-3610
406 994-6367
404 994-4303 (FAX)
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